Light Sensitive Chloramphenicol Analogues.

Chloramphenicol binding site with analogues of chloramphenicol and puromycin.

The effect of a series of puromycin analogues and aminoacyl chloramphenicol derivatives on poly(U,C)-directed polyphenylalanine synthesis in an Escherichia coli cell-free system was examined. A comparison between the structures and activities of the puromycin and chloramphenicol analogues was made to examine the proposal that ribosomal binding sites for both antibiotics overlap. Our results sug...

Chloramphenicol.

Ultrafast light-induced response of photoactive yellow protein chromophore analogues.

The fluorescence decays of several analogues of the photoactive yellow protein (PYP) chromophore in aqueous solution have been measured by femtosecond fluorescence up-conversion and the corresponding time-resolved fluorescence spectra have been reconstructed. The native chromophore of PYP is a thioester derivative of p-coumaric acid in its trans deprotonated form. Fluorescence kinetics are repo...

Multi-antibiotic resistant brain abscess sensitive only to chloramphenicol: a case report

INTRODUCTION A brain abscess is a focal, intracerebral infection that begins as a localized area of cerebritis and develops into a collection of pus surrounded by a well-vascularized capsule. CASE PRESENTATION An 18 year old male was diagnosed to have culture-negative bilateral subdural empyema, which was drained and the patient was discharged, only to return 3 months later with a left tempor...

Amino Acidsinto the Proteins ofMitochondrial Ribosomes from Neurospora crassa Sensitive to Cycloheximide and lnsensitive to Chloramphenicol

Radioactive amino acids were incorporated in vivo into N eurospora crassa cells, and the mitochondrial ribosomes were isolated. The incorporation of radioactivity into the proteins of these ribosomes was inhibited by cycloheximide, but not by chloramphenicol. It is therefore concluded that these proteins are synthesized on the cycloheximide sensitive and chloramphenicol insensitive cytoplasmic ...